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Optimizing UHPLC Fittings and Connections: A Case Study

Eduard Rogatsky, Shaynah B, Cai M and Daniel TS

We experienced persistent problems in achieving robust connections in UHPLC applications utilizing sub-3 micron particle size reversed phase chromatographic columns. Our application involved column testing, which involves frequent disconnection and re-connection of different columns into/from the flow path ofthe UHPLC system. In this paper we report our findings and recommendations.