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A Simple Analytical Method to Determine Alginic Acid, Laminarin and Mannitol in Seaweed Extracts Fertilisers

Silvia Valverde, Lourdes Hernández-Apaolaza, Juan J. Lucena

An analytical method to simultaneously determine Alginic acid, Laminarin and Mannitol in seaweed extracts applied as fertilisers was developed using ultra High-Performance Liquid Chromatography (HPLC) coupled with a Refractive Index Detector (RID). A simple sample treatment for liquid and solid products is proposed involved, dilution with ultrapure water, added glycerol as Internal Standard (IS) and centrifugation step. Chromatographic analysis (25 min) was performed on a Bio-Rad Aminex HPX-87H column and a mobile phase consisting of 0.05% acetic acid solution at a flow rate of 0.5 ml/min. The method was fully validated in terms of selectivity, Limits of Detection (LOD) and Limits of Quantification (LOQ), linearity, recovery, precision and robustness. Finally, the proposed method was successfully applied to analyze the three analytes in different commercial samples. This method was demonstrated to be an innovative tool in the quality control and investigation of these analytes in seaweed fertilizers in a single run.