जर्नल ऑफ़ क्लिनिकल टॉक्सिकोलॉजी
खुला एक्सेस

अमूर्त

Uptake of Poly-dispersed Carbon Nanotubes Induce Cytotoxic and Cytostatic Effect on Human B Cell Leukemia Cells

Babu Mia, Deepam Pushpam, Sameer Bakhshi, Rajiv K. Saxena

Uptake of Fluorescence-tagged Acid-functionalized Single-walled Carbon Nanotubes (FAF-SWCNTs) was examined in Peripheral Blood Derived Mononuclear Cells (PBMC) preparations derived from six patients of Acute B-lymphocytic Leukemia (ALL), five patients of Chronic B-lymphocytic Leukemia (CLL), and four healthy control blood donors. Mean percentage of B cells in PBMC samples was 14.08% (healthy controls), 56.50% (ALL), and 88.74% (CLL). PBMCs were incubated with FAF-SWCNTs for 24 h and uptake in CD19+ B cells was examined by flow cytometry. In all cases, significant uptake of FAF-SWCNTs was found in all CD19+ B cells. A subpopulation of B cells (6% in ALL samples, 3% in CLL samples) showed significantly greater uptake of FAF-SWCNTs. Cellular distribution of the FAF-SWCNTs internalized by B cells in different samples was also examined by confocal microscopy. All B cells internalized the FAF-SWCNTs that were essentially localized in the cytoplasmic region. Incubation of PBMCs with AF-SWCNTs resulted in a significant induction of Phosphatidyl Serine (PS) in ALL and CLL samples but not in PBMCs from healthy donors. Cell cycling studies by using PI staining followed by flow cytometric analysis indicated that the treatment with AF-SWCNTs caused a marked decrease in the S phase of cell cycle in ALL and CLL samples. These results suggest that the treatment with AF-SWCNTs induce cytotoxic and cytostatic effect on human B cell leukemia cells.