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Synthesis of AgNPs using Aspergillus terreus and Evaluation of Its Antimicrobial Activity

Nida Tabassum Khan and Saad Umer Abdul Rehman

Extracellular mycosynthesis of Ag Nano crystals by using the biological organism Aspergillus terreus is one of the most environmental friendly approaches as Aspergillus terreus can easily be cultured on simple media nutrients and shows high metal tolerance. Downstream processing for recovery of extracellular Ag nanocrystals is simpler making the process commercially feasible. Silver nanoparticles fabrication was characterized using UV-visible spectrophotometer and maximum absorbtion was obtained at 450 nm. Nitrate reductase test was used to confirm the presence of reductase protein involved in Ag nanoparticles fabrication. These mycosynthesized nanoparticles were found to have broad antimicrobial spectrum against pathogens including C. albicans, C. tropicalls, C. parapsilosis, C. glabrata, C. krusei, A. flavus, E. coli, P. aeruginosa and S. aureus as determined by agar diffusion method.