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SIMPLE UV SPECTROPHOTOMETRIC ASSAY OF NEW FORMULATION GENTAMYCIN

Safila Naveed, Shabana Naz Shah, Fatima Qamar , Nimra Waheed, and Safeena Nazeer

Gentamycin belongs to Aminoglycoside class of antibiotics.s A simple efficient least time consuming spectrophotometric technique for the assay of Gentamycin has been developed. The assay is based on the ultraviolet UV absorbance maxima at about 202 wavelength of Gentamycin using water as solvent. A sample of drug was dissolved in water to produce a solution containing Gentamycin. Similarly, various dilutions were made. The absorbance of sample preparation was measured at 202nm against the solvent blank and the assay was determined. In our study a simple and quick assay method using U.V spectrophotometer have been developed. The assay is based on measuring the absorbance of new formulation of Gentamycin dilutions at the wavelength of 202 nm. Four different dilutions of 200ppm,100ppm,50ppm and 25ppm is prepared. Their percent assay is calculated and regression equation and regression line is obtained to predict further availability of drug.