बायोकैमिस्ट्री और फार्माकोलॉजी: ओपन एक्सेस
खुला एक्सेस

अमूर्त

Opposite Effects of Monocarboxylic and Dicarboxylic Acids on Osmotic Fragility in Isolated Pig Red Blood Cells: Comparative Study of Membrane Response to Carboxylic Acids Demonstrated in Red Blood Cells of other Mammalian Species

Hitoshi Mineo* and Masaharu Moriyoshi

Lignocellulolytic enzymes are extracellular biocatalysts secreted by filamentous fungi and are involved in the breakdown of recalcitrant lignocellulose plant materials into useful products necessary for fungal growth and development. Even though several studies on filamentous fungi have reported the impact of different substrates on lignocellulolytic enzymes, there is limited information on how mushroom supplements affect secretion of the enzymes, growth, and yield of Pleurotus ostreatus when using the alkaline treatment method. In this study, we investigated the influence of cornmeal (T1) and coffee grounds (T2) supplements on lignocellulosic enzymes at different growth stages and the production of P. ostreatus. We found that lignolytic enzyme activity was significantly higher in the control (CK) and T2 during mycelial stages, while CK had the lowest hydrolytic enzyme activity during primordia and fruiting. Unlike T1 which had the best biological efficiency, T2 exhibited significantly higher levels of lignolytic enzymes during mycelial stage, whereas CMCase and xylanase activities were higher in supplemented treatments than in the control during primordia and fruiting. Taken together our results demonstrated that cornmeal and coffee ground supplements reduce mycelium growth rate, enhance the production of hydrolytic enzymes during fruiting, and remarkably increase the yield and protein content of P. ostreatus.