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Indigenous Trypsin: An Underpinning Approach in Clinical Medicine

Charu Batav*, Ragini Gothalwal

Background: Single cell suspension culture is the prime requirement for cell culture studies as that in clinical medicine. For disaggregating cells, use of enzyme: trypsin has been the first choice by researchers. Commercially, trypsin is obtained from bovine source which adds to the cost of the experiment. Present investigation highlights on utilization of fish waste derived trypsin as a cell dissociating agent anticipated to reduce environmental footprint.

Methods: The current study thus aimed at studying the efficacy of fish waste derived trypsin over commercial counterpart as a cell-dissociating agent. The enzyme isolated from Catla catla visceral waste was purified by Gel filtration chromatography, and characterized by peptide analysis BLAST analysis. The efficacy in terms of cell viability was checked over cancerous cell lines procured from NCCS, Pune, India.

Results: The crude enzyme extract isolated from Catla catla visceral waste exhibited 145.22 mg.ml^-1 protein content which was enhanced to 152.93 mg.ml^-1 after purification. Upon performing BLAST analysis of the peptides, the enzyme was found to be trypsin. The enzyme (0.1% and 1% concentration) retained 90% cell viability at 10 sec when compared with bovine source.

Conclusion: The fish visceral waste was considered as a novel source of trypsin enzyme which demonstrated an endurable cell dissociating ability.