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अमूर्त

High Performance Liquid Chromatography, TLC Densitometry, Firstderivative and First-derivative ratio spectrophotometry for de-termination of Rivaroxaban and its alkaline Degradates in Bulk Powder and its Tablets

Lories IB, Mostafa AA and Girges MA

Four sensitive and selective stability indicating methods for the determination of rivaroxaban (RIV) were developed. Method a was an isocratic RP-HPLC, good resolution between peaks corresponding to the degradates from analyte was achieved on C18 column. The mobile phase is 1.2% w/v potassium dihydrogen phosphate pH 3.5 ± 0.2 and acetonitrile (70:30, v/v). The detection was carried out at 280 nm. Method B depends on quantitative densitometric determination of thin layer chromatography TLC of rivaroxaban in the presence of it’s degradates without any interference. The developing system was chloroform-isobutyl alcohol (50:50 v/v).The chromatogram was scanned at 280 nm. Method C was based on the first derivative (D1) measurement of the drug at 237.4 nm; zero contribution point of its alkaline degradates. Method D was based on the resolution of the drug and its alkaline degradates by first derivative ratio spectra (DD1 successfully applied for the determination of rivaroxaban in bulk powder, pharmaceutical formulation and in presence of its alkaline degradates. The obtained results were statistically analyzed and compared with those obtained by the reported method