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EFFECT OF DICLOFENAC SODIUM ANGIOGENESIS USING CHORIOALLANTOIC MEMBRANE (CAM) ASSAY

Iradat Hussain , Muhammad Ovais Omer, Muhammad Ashraf, Habib-Ur-Rehman

Angiogenesis, the formation of new blood vessels, is a hallmark of almost all neoplastic and non-neoplastic degenerative diseases. This process supports normal physiology as well as it contributes in progression of different diseases. Angiogenesis contributes in growth of tumor and progressive arthritis. Inflammatory mediators are involved in cancer induced angiogenic process. Cyclo-oxygenases promote these mediators which help in cell migration and endothelial cell spreading. To explore the role of diclofenac sodium in angiogenesis we have used in vitro Chorioallantoic membrane assay. A novel image probing system SPIP (scanning probe image processor) was utilized for assessment and quantification of structural changes in CAMs. Fourteen parameters of 3D surface roughness were also evaluated for quantification. Application of diclofenac sodium on Chorioallantoic membrane at day six of incubation (0.7% concentration of diclofenac sodium) showed anti-angiogenic effect. Results showed marked changes in architecture of CAMs, thinning of primary, secondary and tertiary blood vessels, reduction in surface roughness parameters, increase in kurtosis of surface, and decrease in Abbott curve. The substantial quantities of diclofenac sodium use locally may exhibit anti-angiogenic activity in the same manner those seen in in-vitro and explain its clinical efficacy.