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DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BRIMONIDINE TARTRATE AND TIMOLOL MALEATE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Hani M.Hafez, Abdullah A. Elshanawane, Lobna M. Abdelaziz, Mustafa S. Mohram

Brimonidine Tartrate and Timolol Maleate are used in treatment of glaucoma by decreasing intra ocular pressure. A Validated HPLC method was developed for the assay of them. The method was performed on BDS HYPERSIL Cyano column (250x4.6 mm, 5μ) and the mobile phase consisted of Ammonium acetate (pH 5.0, 0.01M) - Methanol (40:60, V/V) which pumped at a flow rate equals to 1.5 ml/min at ambient temperature. 20 μl of drugs sample solutions were monitored at two fixed wavelengths (lambda = 254.0 nm for Brimonidine Tartrate and 300.0 nm for Timolol Maleate). The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation according to ICH.