आंतरिक चिकित्सा: खुली पहुंच

आंतरिक चिकित्सा: खुली पहुंच
खुला एक्सेस

आईएसएसएन: 2165-8048


Co-regulated Intestinal c-kit by Interleukin-17 and Interleukin-10 on Post-infectious Irritable Bowel Syndrome in Mice

Bo Yang, Xuesong Bai, Yijun Yu, Honglan Liu, Cheng Lan, Dan Liu, Xuchun Zhou


The aim of this study was to investigate the probable immune mechanisms of PI-IBS.


C57L/B6 mice, which have been infected by 500 Trichinella larvae for 56 days, were used to PI-IBS model mice, the levels of the intestinal mRNAs and proteins of c-kit, interleukin-10 (IL-10), and IL-17 were measured by RTPCR and western blotting or ELISA. The locations of c-kit were detected using immunohistochemical staining.


Levels of the c-kit protein and mRNA were up-regulated in the whole PI-IBS mice intestine. Compared to the control bowel segments, IL-17 levels were significantly higher in the duodenum and ileum, and IL-10 levels were lower in jejunum, ileum, and colon. By immunohistochemical staining, the increased signals of the c-kit were mainly detected in the submucosa and myenteron.


These results suggested that increased levels of the c-kit resulted in the changes of the intestinal motility and visceral sensitivity in PI-IBS mouse model induced by Trichinella Spiralis infection, and which may be coregulated by IL-17 and IL-10 in pathophysiology of PI-IBS.