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A novel interplay between intestinal bacteria and metabolites in IgA nephropathy identified via integrated microbiome and metabolome approaches

Hongwei W

Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis. Intestinal bacteria and their metabolites have been implicated in various diseases. Improved understanding of the gut microbiota and its metabolic capabilities will facilitate development of diagnostic, therapeutic, and prognostic methods for IgAN. We identified gut microbiota and metabolite biomarkers of IgAN by analyzing microbiomes and metabolomes of fecal and serum samples of IgAN patients and healthy controls using 16s ribosomal RNA gene sequencing and liquid chromatography-tandem mass spectrometry, respectively, and bioinformatics approaches. We found that relative abundances of Streptococcus and Enterococcus were higher in IgAN patients, whereas Bacteroidetes and Bacteroides were lower. The changes in gut microbiota affected metabolism and absorbance of microbiota-associated metabolites of IgAN patients, in particular polyunsaturated fatty acids, free amino acids and oligopeptides, and activated the phenylalanine metabolism pathway. Also, 5hydroxyeicosatetraenoic acid and 5-hydroxy6E,8Z,11Z-eicosatrienoic acid were proved to be associated with the classification of segmental glomerular sclerosis but not 24h urine protein and  
 
 
 
 
estimated glomerular filtration rate. Our findings demonstrate an interplay between intestinal bacteria  
 
 
and metabolites in IgAN. The identified metabolites may have diagnostic and therapeutic applications. 
 
16th World Nephrology Conference August 20-21, 2020 Webinar 
 
Biography: 
 
Hongwei Wu is doing his PhD study in Jinan University. He is the doctor of The First Affiliated Hospital of Jinan University and major in nephritis. He has published 3 papers in professional journals.