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Electro Analytical Method for Detection of Bacteria Using Amoxicillin Modified Carbon Paste Electrode: Analytical Application in Milk

Koffi OFAB, Avo Bile BE, El Amraoui R, Abdelaoui O and Chtaini A

A carbon paste electrode modified with amoxicillin antibiotic was employed for the quantification of bacteria from aqueous solutions. The new electrode (AMX-CPE) revealed interesting electroanalytical detection of Staphylococcus aureus based on the adsorption of bacteria onto amoxicillin under open circuit conditions. The influence of variables such as the accumulation time, pH solution and optical density (OD) was tested by square wave voltammetry (SWV) and cyclic voltammetry (CV). The response depends on the optical density of bacteria in the bulk solution as well as the parameters involved in the preconcentration, pH and the measurement steps. The elaborate electrode was used for the detection of S. aureus in milk and showed a very good limit of detection (3.00 × 10-6) in milk.