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Anti-Cancer Drug Assay for Micro-Ellipse Filters after Sensitively Capturing Circulating Tumor Cells

Hongmei Chen, Baoshan Cao, Hongda Chen, Hui Yang1, Peihua Liu, Tengfei Wang and Chunfei Hu

Circulating tumor cells (CTCs) carrying significant information from primary tumor rendering precise enumeration valuable. Extremely rare number detected in the patient sample belongs to early stage of cancer. Detection, characterization and further molecular analysis determine prognosis and diagnosis. In this work, we performed Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) to precisely enumerate number of tumor cells captured on the highly-sensitive gradual narrowed micro-ellipse filters. Clinical assay was performed on Ellipse filters to assess further validity in non-small-cell-lung cancer (NSCLC) patient blood with CTCs detected positive. Antidrug cancer assays were performed with micro-ellipse filters after cells captured on the chip treated with baicalein (BAE) of different concentrations. Highly-sensitive micro-ellipse filters validate efficiency of clinical enumeration of metastatic patient samples, anti-cancer drug responses after improvement of co-culture design and mutational biological molecular analysis.